BORRELIOOSIN TUTKIMUSMENETELMISTÄ

[Bb]

Lähettäjä: Soijuv Lähetetty: 10.1.2005 18:53

Keskustelin Brorsonin kanssa hänen mielipidettään parhaasta menetelmästä jolla borrelioosi voidaan todeta verestä. Seuraavassa hänen vastauksensa.

O. Brorsonin mielestä Bb voidaan löytää verestä todennäköisesti parhaiten elektronimikroskopian avulla:


"The best method to discover B.b from blood probably is the method used by Dagmar Hulinska. She is using immuno-capture electron miroscope technique. She has pretty goood results."


Hän on itse käyttänyt Igenexin Western Blot menetelmää kahden vuoden ajan. Potilaiden vasta-ainepitoisuudet ovat muuttuneet hoitojen aikana seuraavasti:


I have been doing sequential IGeneX Western blots for about two years. With treatment, the sequence is usually as follows:

IgM Pos/IgG Pos --> IgM Pos/IgG Neg --> IgM Equivocal/IgG Neg --> IgM Neg/IgG Neg

Vaihteluita on esiintynyt jonkin verran. Yleensä IgM on pysynyt IgG:tä kauemmin positiivisena:


There are variations on this theme, but the disappearance of positive testing usually correlates with improved symptoms. In general, the IgM response is more durable than the IgG response.


Hulinskan tutkimus:


Cent Eur J Public Health. 1993 Dec;1(2):81-5.


Electron microscopy and the polymerase chain reaction of spirochetes from the blood of patients with Lyme disease.

Hulinska D, Krausova M, Janovska D, Rohacova H, Hancil J, Mailer H.

Department of Electron Microscopy, National Institute of Public Health, Prague, Czech Republic.

Results of studies using direct antigen detection suggest that seronegative Lyme borreliosis is not rare and support the hypothesis that Borrelia antigens can persist in humans. We report three successful cultures from blood out of 30 attempts from 96 Lyme disease patients. The proof of borreliaemia in early or late phases of Lyme disease by immuno-capture electron microscopy has practical importance for subsequent cultivation. The polymerase chain reaction with oligonucleotide sequences directed against 16S rRNA identified two of our blood isolates as Borrelia burgdorferi genospecies III., VS 461 group, and one as Borrelia garinii sp. nov. All of the three isolates were reactive with monoclonal antibody H9724 against flagellin and with antibody against main extracellular protein at 83 kDa. Borrelia garinii had a single predominant protein OspA at 33.5 kDa and reacted with monoclonal antibody H5332 in contrast to two isolates of the VS 461 group with two major proteins OspA and OspB at 32.5 and 35 kDa. We conclude that isolation of spirochetes from the blood might prove successful in clinically selected cases of Lyme borreliosis. Immuno-capture electron microscopy has proved to be a sensitive assay for monitoring and studying Lyme borreliosis.

PMID: 8004045 [PubMed - indexed for MEDLINE]