BORRELIOOSITESTI ELISA

[Bb]

Lähettäjä: Soijuv Lähetetty: 30.3.2005 8:03

Borrelioosia epäiltäessä tutkitaan aina vasta-aineiden esiintymistä veressä (Borrelia IgM ja IgG). Italialaisessa tutkimuksessa vertailtiin kolmea kaupallista ELISA-testiä. Tämäkin uusi tutkimus vahvisti lukuisia aiempia tutkimuksia ja kannanottoja siitä että ELISA-testimenetelmä ei ole luotettava. Tutkimuksessa käytettyjen testien herkkyys vaihteli 36,8 % - 70 %:n välillä - eli borrelioosidiagnoosi on edelleen kliininen eikä verikokeisiin yms. perustuva:


Comparative evaluation of three different ELISA methods for the diagnosis of early culture-confirmed *Lyme* disease in Italy.

http://www.ncbi.nlm.nih.gov:80/entrez/q ... s=15770021>
AUTHORS: Antonella Marangoni, Monica Sparacino, Francesca Cavrini, Elisa Storni, Valeria Mondardini, Vittorio Sambri, Roberto Cevenini
AFFILIATION: Sezione di Microbiologia - DMCSS, University of Bologna, St Orsola Hospital, via Massarenti 9, Bologna, Italy.
REFERENCE: J Med Microbiol 2005 Apr 54(Pt 4):361-7

In this study the raising and development of the immune response to *Borrelia burgdorferi* infection in 45 Italian patients suffering from culture-confirmed *Lyme* borreliosis erythema migrans was investigated. A total of 95 serially collected serum samples were tested by using three different commercial ELISAs: recomWell Borrelia (Mikrogen), Enzygnost Borreliosis (DADE Behring) and Quick ELISA C6 Borrelia (Immunetics).

The sensitivities of the ELISAs were as follows: Enzygnost Borreliosis IgM , 70.5 %; Quick ELISA C6 Borrelia, 62.1 %; recomWell Borrelia IgM, 55.7 %; recomWell Borrelia IgG, 57.9 %; and Enzygnost Borreliosis IgG, 36.8 %. In order to compare the specificity values of the three ELISAs, a panel of sera obtained from blood donors (210 samples coming from a non- endemic area and 24 samples from an endemic area) was tested, as well as sera from patients suffering from some of the most common biological conditions that could result in false-positive reactivity in *Lyme* disease serology (n = 40). RecomWell Borrelia IgG and recomWell Borrelia IgM were the most specific (97.1 % and 98.9 %, respectively), followed by Quick ELISA C6 Borrelia (96.7 %). Enzygnost Borreliosis IgG and IgM achieved 90.1 % and 92.3 % specificity, respectively. Sera that gave discrepant results when tested by the three ELISAs were further analysed by Western blotting.

PMID: 15770021 <http://refscout.com/cgi-bin/exportAbstr ... d=15770021>