Lähettäjä: Soijuv Lähetetty: 26.12.2005 12:19
Artemisiiniä käytetään mm. babesioosin ja malarian hoitoon. Aineella on havaittu eläinkokeissa olevan myös syöpäsoluja tuhoava vaikutus:
Volume 231, Issue 1, Pages 43-48 (8 January 2006)
Oral artemisinin prevents and delays the development of 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer in the rat
Henry Lai, Narendra P. Singh
Received 30 October 2004; accepted 14 January 2005
Abstract
Artemisinin, a compound isolated from the sweet wormwood Artemisia annua L., has previously been shown to have selective toxicity towards cancer cells in vitro. In the present experiment, we studied the potential of artemisinin to prevent breast cancer development in rats treated with a single oral dose (50mg/kg) of 7,12-dimethylbenz[a]anthracene (DMBA), known to induce multiple breast tumors. Starting from the day immediately after DMBA treatment, one group of rats was provided with a powdered rat-chow containing 0.02% artemisinin, whereas a control group was provided with plain powdered food.
For 40 weeks, both groups of rats were monitored for breast tumors. Oral artemisinin significantly delayed (P<.002) and in some animals prevented (57% of artemisinin-fed versus 96% of the controls developed tumors, P<.01) breast cancer development in the monitoring period. In addition, breast tumors in artemisinin-fed rats were significantly fewer (P<.002) and smaller in size (P<.05) when compared with controls. Since artemisinin is a relatively safe compound that causes no known side effects even at high oral doses, the present data indicate that artemisinin may be a potent cancer-chemoprevention agent.
Keywords: Artemisinin, Daily oral intake, Breast cancer, Prevention
Department of Bioengineering, University of Washington, Box 357962, Seattle,
WA 98195-7962, USA
Corresponding author. Tel.: +1 206 543 1071; fax: +1 206 685 3925.
PII: S0304-3835(05)00072-8
doi:10.1016/j.canlet.2005.01.019
ARTEMISIINI
Valvojat: Jatta1001, Borrelioosiyhdistys, Bb
ARTEMISIINI
Viimeksi muokannut Bb, La Maalis 07, 2009 16:15. Yhteensä muokattu 3 kertaa.
Lähettäjä: Soijuv Lähetetty: 28.12.2005 15:24
Artemisiini näyttäisi seuraavan tutkimuksen mukaan olevan halpa ja tehokas syövänhoitomenetelmä. Artemisiini aikaansaa syöpäsolujen kuoleman.
Anticancer Res. 2004 Jul-Aug;24(4):2277-80.
Artemisinin induces apoptosis in human cancer cells.
Singh NP, Lai HC.
Department of Bioengineering, University of Washington, Seattle, Washington 98195-7962, USA. Narendra@u.washington.edu
BACKGROUND: Artemisinin is a chemical compound extracted from the wormwood plant, Artemisia annua L. It has been shown to selectively kill cancer cells in vitro and retard the growth of implanted fibrosarcoma tumors in rats. In the present research, we investigated its mechanism of cytotoxicity to cancer cells.
MATERIALS AND METHODS: Molt-4 cells, in complete RPMI-1640 medium, were first incubated with 12 microM of human holotransferrin at 37 degrees C in a humid atmosphere of 5% CO2 for one hour. This enhanced the iron supply to the cells. The cells were then pelleted and transferred to a complete RPMI-1640 containing 200 microM of an analog dihydroartemisinin (DHA) and incubation was started (0 h). In addition, some culture samples were treated with holotransferrin alone and some (controls) were assayed without neither holotransferrin nor DHA treatment. Cells were counted and DNA diffusion assay was used to evaluate apoptosis and necrosis in each sample at 0 h and at 1, 2, 4 and 8 h of incubation.
RESULTS: DHA treatment significantly decreased cell counts and increased the proportion of apoptosis in cancer cells compared to controls (chi2=4.5, df=1, p<0.035). Addition of holotransferrin significantly further decreased cell counts (chi2=4.5, df=1, p<0.035) and increased apoptosis (chi2=4.5, df=1, p<0.035). No necrotic cells were observed.
CONCLUSION: This rapid induction of apoptosis in cancer cells after treatment with DHA indicates that artemisinin and its analogs may be inexpensive and effective cancer agents.
PMID: 15330172 [PubMed - indexed for MEDLINE]
Artemisiini näyttäisi seuraavan tutkimuksen mukaan olevan halpa ja tehokas syövänhoitomenetelmä. Artemisiini aikaansaa syöpäsolujen kuoleman.
Anticancer Res. 2004 Jul-Aug;24(4):2277-80.
Artemisinin induces apoptosis in human cancer cells.
Singh NP, Lai HC.
Department of Bioengineering, University of Washington, Seattle, Washington 98195-7962, USA. Narendra@u.washington.edu
BACKGROUND: Artemisinin is a chemical compound extracted from the wormwood plant, Artemisia annua L. It has been shown to selectively kill cancer cells in vitro and retard the growth of implanted fibrosarcoma tumors in rats. In the present research, we investigated its mechanism of cytotoxicity to cancer cells.
MATERIALS AND METHODS: Molt-4 cells, in complete RPMI-1640 medium, were first incubated with 12 microM of human holotransferrin at 37 degrees C in a humid atmosphere of 5% CO2 for one hour. This enhanced the iron supply to the cells. The cells were then pelleted and transferred to a complete RPMI-1640 containing 200 microM of an analog dihydroartemisinin (DHA) and incubation was started (0 h). In addition, some culture samples were treated with holotransferrin alone and some (controls) were assayed without neither holotransferrin nor DHA treatment. Cells were counted and DNA diffusion assay was used to evaluate apoptosis and necrosis in each sample at 0 h and at 1, 2, 4 and 8 h of incubation.
RESULTS: DHA treatment significantly decreased cell counts and increased the proportion of apoptosis in cancer cells compared to controls (chi2=4.5, df=1, p<0.035). Addition of holotransferrin significantly further decreased cell counts (chi2=4.5, df=1, p<0.035) and increased apoptosis (chi2=4.5, df=1, p<0.035). No necrotic cells were observed.
CONCLUSION: This rapid induction of apoptosis in cancer cells after treatment with DHA indicates that artemisinin and its analogs may be inexpensive and effective cancer agents.
PMID: 15330172 [PubMed - indexed for MEDLINE]