Lähettäjä: Soijuv Lähetetty: 14.11.2005 15:47
Babesioosin aiheuttajaa (babesia microti) on löydetty Tsekeistä!
http://www.ncbi.nlm.nih.gov/entrez/quer ... uery_hl=11
Folia Parasitol (Praha). 2005 Sep;52(3):274-6.
Babesia microti (Piroplasmida: Babesiidae) in nymphal Ixodes ricinus (Acari: Ixodidae) in the Czech Republic.
Rudolf I, Golovchenko M, Sikutova S, Rudenko N, Grubhoffer L, Hubalek Z.
Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic. rudolf@ivb.cz
A total of 350 nymphs of the common tick Ixodes ricinus (Linnaeus, 1758) were collected in an endemic focus of Lyme borreliosis (South Moravia, Czech Republic) and examined for the presence of the protozoan Babesia microti (Franca, 1909) by polymerase chain reaction (PCR), using primers specific for the B. microti gene encoding small subunit rRNA. The assay revealed five positive pools (out of 70 pools examined); the corresponding prevalence rate was about 1.5%. Sequence analysis of the PCR products confirmed their 100% homology with that of B. microti. The study represents the first evidence of B. microti in ixodid ticks in the Czech Republic.
PMID: 16270809 [PubMed - in process]
BABESIOOSIN AIHEUTTAJA TSEKEISSÄ
Valvojat: Jatta1001, Borrelioosiyhdistys, Bb
BABESIOOSIN AIHEUTTAJA TSEKEISSÄ
Viimeksi muokannut Bb, La Maalis 07, 2009 14:51. Yhteensä muokattu 1 kertaa.
Lähettäjä: Soijuv Lähetetty: 23.1.2006 8:49
73-vuotias mies kuoli babesioosiin. Miehelle oli tehty aiemmin pernanpoisto. Hänen vasta-aineensa olivat voimakkaasti positiiviset Babesia divergensiä kohtaan joka aiheuttaa babesioosia eläimille ja ihmisille nimenomaan Euroopassa.
Ann Intern Med. 1996 Apr 1;124(7):643-50.
Comment in:
Ann Intern Med. 1997 Jan 15;126(2):172.
A fatal case of babesiosis in Missouri: identification of another piroplasm that infects humans.
Herwaldt B, Persing DH, Precigout EA, Goff WL, Mathiesen DA, Taylor PW,
Eberhard ML, Gorenflot AF.
Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
OBJECTIVE: To characterize the etiologic agents (MO1) of the first reported
case of babesiosis acquired in Missouri. DESIGN: Case report, serologic
testing, animal inoculations, and molecular studies. SETTING: Southeastern
Missouri.
PATIENT: A 73-year-old man who had had a splenectomy and had a
fatal case of babesiosis. MEASUREMENTS: Serum specimens from the patient
were assayed by indirect immunofluorescent antibody testing and
immunoprecipitation for reactivity with antigens from various Babesia
species. Whole blood obtained from the patient before treatment was
inoculated into hamsters and jirds and into calves and bighorn sheep that
had had splenectomy and were immunosuppressed with dexamethasone.
Piroplasm-specific nuclear small-subunit ribosomal DNA was recovered from
the patient's blood by using broad-range amplification with the polymerase
chain reaction; a 144 base-pair region of the amplification product was
sequenced; and phylogenetic analysis was done to compare MO1 with various
Babesia species.
RESULTS: Indirect immunofluorescent antibody testing showed
that the patient's serum had strong reactivity with Babesia divergens, which
causes babesiosis in cattle and humans in Europe, but that it had minimal
reactivity with B. microti and WA1, which are the piroplasms previously
known to cause zoonotic babesiosis in the United States.
Immunoprecipitations showed that MO1 is more closely related to B. divergens
than to B. canis (a canine parasite). None of the experimentally inoculated
animals became demonstrably parasitemic. Phylogenetic analyses, after DNA
sequencing, showed that MO1 is most closely related to B. divergens (100%
similarity).
CONCLUSIONS: Although MO1 is probably distinct from B.
divergens, the two share morphologic, antigenic, and genetic
characteristics; MO1 probably represents a Babesia species not previously
recognized to have infected humans. Medical personnel should be aware that
patients in the United States can have life-threatening babesiosis even
though they are seronegative to B. microti and WA1 antigen.
Publication Types:
Case Reports
PMID: 8607592 [PubMed - indexed for MEDLINE]
73-vuotias mies kuoli babesioosiin. Miehelle oli tehty aiemmin pernanpoisto. Hänen vasta-aineensa olivat voimakkaasti positiiviset Babesia divergensiä kohtaan joka aiheuttaa babesioosia eläimille ja ihmisille nimenomaan Euroopassa.
Ann Intern Med. 1996 Apr 1;124(7):643-50.
Comment in:
Ann Intern Med. 1997 Jan 15;126(2):172.
A fatal case of babesiosis in Missouri: identification of another piroplasm that infects humans.
Herwaldt B, Persing DH, Precigout EA, Goff WL, Mathiesen DA, Taylor PW,
Eberhard ML, Gorenflot AF.
Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
OBJECTIVE: To characterize the etiologic agents (MO1) of the first reported
case of babesiosis acquired in Missouri. DESIGN: Case report, serologic
testing, animal inoculations, and molecular studies. SETTING: Southeastern
Missouri.
PATIENT: A 73-year-old man who had had a splenectomy and had a
fatal case of babesiosis. MEASUREMENTS: Serum specimens from the patient
were assayed by indirect immunofluorescent antibody testing and
immunoprecipitation for reactivity with antigens from various Babesia
species. Whole blood obtained from the patient before treatment was
inoculated into hamsters and jirds and into calves and bighorn sheep that
had had splenectomy and were immunosuppressed with dexamethasone.
Piroplasm-specific nuclear small-subunit ribosomal DNA was recovered from
the patient's blood by using broad-range amplification with the polymerase
chain reaction; a 144 base-pair region of the amplification product was
sequenced; and phylogenetic analysis was done to compare MO1 with various
Babesia species.
RESULTS: Indirect immunofluorescent antibody testing showed
that the patient's serum had strong reactivity with Babesia divergens, which
causes babesiosis in cattle and humans in Europe, but that it had minimal
reactivity with B. microti and WA1, which are the piroplasms previously
known to cause zoonotic babesiosis in the United States.
Immunoprecipitations showed that MO1 is more closely related to B. divergens
than to B. canis (a canine parasite). None of the experimentally inoculated
animals became demonstrably parasitemic. Phylogenetic analyses, after DNA
sequencing, showed that MO1 is most closely related to B. divergens (100%
similarity).
CONCLUSIONS: Although MO1 is probably distinct from B.
divergens, the two share morphologic, antigenic, and genetic
characteristics; MO1 probably represents a Babesia species not previously
recognized to have infected humans. Medical personnel should be aware that
patients in the United States can have life-threatening babesiosis even
though they are seronegative to B. microti and WA1 antigen.
Publication Types:
Case Reports
PMID: 8607592 [PubMed - indexed for MEDLINE]
Viimeksi muokannut Bb, La Maalis 07, 2009 14:52. Yhteensä muokattu 1 kertaa.
Lähettäjä: Soijuv Lähetetty: 14.2.2006 9:25
Pietarissa esiintyy babesiaa, ehrlichiaa ja muita punkkien välittämiä taudinaiheuttajia!
Pietarin alueella tutkittiin esiintyykö punkeissa (1200 kpl) borreliaa, ehrlichiaa, puutiaisaivokuumeen aiheuttajaa, babesiaa. Löydös: 417 punkkia oli infektoitunut yhdellä tai useammalla taudinaiheuttajalla. Babesiaa löytyi seitsemästä punkista ja ne esiintyivät aina yhdessä borreliabakteerin kanssa (garinii ja/tai afzelii). Borrelia spp (toisintokuume) esiintyi 34 % :ssa punkkeja - yhdessä ehrlichioosin kanssa 6,2 % ja babesian kanssa 0.9 %. Artikkelin mukaan mitä useamman taudinaiheuttajan saa, sitä vaikeammat oireet.
Suomessa ei kuitenkaan edelleenkään yleensä tutkita kuin borreliavasta- aineet (borrelia burgdorferi).
Exp Appl Acarol. 2003;29(3-4):345-53.
Evidence of Babesia microti infection in multi-infected Ixodes persulcatus
ticks in Russia.
Alekseev AN, Semenov AV, Dubinina HV.
Zoological Institute, Russian Academy of Sciences, Universitetskaya nab., 1, 199034 St. Petersburg, Russia. devana@HD1389.spb.edu
To detect Babesia-infected Ixodes persulcatus Shulze in a suburb of St. Petersburg, Russia, 738 adult ticks were studied using Babesia specific primers and PCR techniques. The entire sample (more than 1,200 individuals) was screened for the presence of Borrelia spp., Ehrlichia spp. and tick-borne encephalitis virus (TBEV).
All 7 ticks infected with Babesia microti, were also infected with other pathogens (all 7 among 417 infected ticks, zero amongst the remaining 321 naive ones (chi2 = 5.25, p<0.05). Babesia microti occurred twice with Borrelia afzelii, 3 times with Borrelia garinii, once with both, and once with both B. garinii and TBEV.
The prevalence of infection with Borrelia spp. was 34.0%, with Ehrlichia spp. 6.2%, with TBEV 1.5%, and with Ba. microti 0.9%. Babesia microti infection was not found in combination with Ehrlichia sp. or Borrelia burgdorferi sensu stricto. The latter pathogen (prevalence 2.6%), just like Ba. microti, was not encountered as a monoinfection. The data suggest that Ba. microti infection can only survive in I. persulcatus in combination with Borrelia spp. (7 of 7 infections).
The disease in humans is more severe and longer-lasting when more than one pathogen is involved. Our observations show that the well known St. Petersburg focus of tick-borne encephalitis and Lyme disease is also a focus of ehrlichiosis and babesiosis.
PMID: 14635819 [PubMed - indexed for MEDLINE]
Pietarissa esiintyy babesiaa, ehrlichiaa ja muita punkkien välittämiä taudinaiheuttajia!
Pietarin alueella tutkittiin esiintyykö punkeissa (1200 kpl) borreliaa, ehrlichiaa, puutiaisaivokuumeen aiheuttajaa, babesiaa. Löydös: 417 punkkia oli infektoitunut yhdellä tai useammalla taudinaiheuttajalla. Babesiaa löytyi seitsemästä punkista ja ne esiintyivät aina yhdessä borreliabakteerin kanssa (garinii ja/tai afzelii). Borrelia spp (toisintokuume) esiintyi 34 % :ssa punkkeja - yhdessä ehrlichioosin kanssa 6,2 % ja babesian kanssa 0.9 %. Artikkelin mukaan mitä useamman taudinaiheuttajan saa, sitä vaikeammat oireet.
Suomessa ei kuitenkaan edelleenkään yleensä tutkita kuin borreliavasta- aineet (borrelia burgdorferi).
Exp Appl Acarol. 2003;29(3-4):345-53.
Evidence of Babesia microti infection in multi-infected Ixodes persulcatus
ticks in Russia.
Alekseev AN, Semenov AV, Dubinina HV.
Zoological Institute, Russian Academy of Sciences, Universitetskaya nab., 1, 199034 St. Petersburg, Russia. devana@HD1389.spb.edu
To detect Babesia-infected Ixodes persulcatus Shulze in a suburb of St. Petersburg, Russia, 738 adult ticks were studied using Babesia specific primers and PCR techniques. The entire sample (more than 1,200 individuals) was screened for the presence of Borrelia spp., Ehrlichia spp. and tick-borne encephalitis virus (TBEV).
All 7 ticks infected with Babesia microti, were also infected with other pathogens (all 7 among 417 infected ticks, zero amongst the remaining 321 naive ones (chi2 = 5.25, p<0.05). Babesia microti occurred twice with Borrelia afzelii, 3 times with Borrelia garinii, once with both, and once with both B. garinii and TBEV.
The prevalence of infection with Borrelia spp. was 34.0%, with Ehrlichia spp. 6.2%, with TBEV 1.5%, and with Ba. microti 0.9%. Babesia microti infection was not found in combination with Ehrlichia sp. or Borrelia burgdorferi sensu stricto. The latter pathogen (prevalence 2.6%), just like Ba. microti, was not encountered as a monoinfection. The data suggest that Ba. microti infection can only survive in I. persulcatus in combination with Borrelia spp. (7 of 7 infections).
The disease in humans is more severe and longer-lasting when more than one pathogen is involved. Our observations show that the well known St. Petersburg focus of tick-borne encephalitis and Lyme disease is also a focus of ehrlichiosis and babesiosis.
PMID: 14635819 [PubMed - indexed for MEDLINE]