UUSI TUTKIMUSMENETELMÄ

[Bb]

Lähettäjä: Soijuv Lähetetty: 4.11.2005 8:21

Mielenkiintoinen tutkimus! Puolalaisessa tutkimuksessa selvitettiin tiettyjen tulehdusreseptoreiden (ICAM 1-3) määrää neuroborrelioosia ja puutiaisaivokuumetta sairastavien veressä ja selkäydinnesteessä.

Kummassakin ryhmässä havaittiin kohonneita arvoja. Ne osoittavat lymfosyyttien aktiivisuutta aivokalvoissa ja ovat merkkinä tulehdusprosessista. Veren ICAM 1 ja 2 määrät olivat neuroborrelioosia sairastavilla selkeästi koholla. Se saattaa osoittaa neuroborrelioosin olevan systeeminen tulehdussairaus. ICAM 2:n määrä selkäydinnesteessä laski hoidon jälkeen neuroborrelioosia sairastavilla verrattuna aivokuumetta sairastaviin.


http://www.ncbi.nlm.nih.gov/entrez/quer ... query_hl=3

Pol Merkuriusz Lek. 2005 Aug;19(110):152-7.

Serum and cerebrospinal concentrations of sICAM-1 sICAM-2, sICAM-3 in neuroborrellosis and tick borne encephalitis--preliminary report

[Article in Polish]

Zajkowska JM, Izycka A, Jablonska E, Hermanowska-Szpakowicz T, Kondrusik M, Pancewicz S, Grygorczuk S, Swierzbinska R.

Klinika Chorob Zakaznych i Neuroinfekcji Akademii Medycznej, Bialymstoku. zajkowsk@kki.pl

OBJECTIVE: The aim was to evaluate the concentrations of the soluble forms of ICAM-1, ICAM-2, ICAM-3 in the serum and cerebrospinal fluid (CSF) of patients with neuroborreliosis and thick borne encephalitis before and after therapy in comparison with the control group.

MATERIAL AND METHODS: We examined 30 patients, 10 in each group: neuroborreliosis-group I (NB), tick borne encephalitis--group II (TBE) and in the control group (group K). The diagnosis of neuroborreliosis and TBE based on the clinical features was confirmed by ELISA assays: FSME Virus/TBEVirus test (VIRION, Germany) for TBE and Borrelia IgM and IgG Recombinant (Biomedica Austria) for NB. The assays of sICAM-1, sICAM-2, sICAM-3 (ELISA, Bender Med System, USA) were performed twice in group I and II: before and after 3-4 weeks long treatment and once in control group.

RESULTS AND CONCLUSIONS: Increased concentration of soluble forms of ICAM-1, ICAM-2, ICAM-3 in CSF suggest their important role in inflammatory process of viral and bacterial origin. In NB group, the serum concentrations of sICAM-1, sICAM-2 were significantly increased before and after treatment in comparison with control as well as with the analogous test results in TBE group. It may suggest NB as the part of systemic inflammation.

The CSF concentration of sICAM-2 decreases after treatment in NB group in comparison with the analogous test results of TBE group. The increased CSF concentration of sICAM-2 in TBE group when compared to the CSF concentration in NB group suggest slow recovery and still persisting immunological activation in this group, even when the neurological symptoms disappeared. Increased concentrations in CSF in both diseases indicate on intrameningeal activity of lymphocytes and may be an useful marker of inflammation.

PMID: 16245421 [PubMed - in process]