NEUROBORRELIOOSI

[Bb]

Lähettäjä: Soijuv Lähetetty: 2.1.2006 11:58

Borrelia burgdorferin tunkeutuminen keskushermostoon
on luultua yleisempää. Neuroborrelioosi ei liene harvinainen
ilmiö Pohjois-Amerikassakaan:


Neurology. 1995 Jan;45(1):165-72.
Inoculation of nonhuman primates with the N40 strain of Borrelia
burgdorferi leads to a model of Lyme neuroborreliosis faithful to the human
disease.

Pachner AR, Delaney E, O'Neill T, Major E.

Department of Neurology, Georgetown University Medical Center,
Washington, DC.

We injected rhesus macaques with a highly infective strain of Borrelia
burgdorferi to assess whether experimentally inoculated nonhuman primates
(NHPs) could serve as models of human Lyme neuroborreliosis (LNB). The
animals developed biopsy-confirmed erythema migrans in the area of the
inoculations. ELISA testing of sera revealed strong antibody reactivity to B
burgdorferi antigens, and Western blotting showed that 16-, 22-, 31-, 34-,
and 41-kd proteins of the spirochete were major antigens recognized by
antibody. Culture and polymerase chain reaction (PCR) testing of serial CSF
specimens revealed that chronic infection of the CNS occurred in all NHPs
injected. CSF pleocytosis occurred concurrently with CNS infection. Brain
MRI revealed intense meningeal inflammation in one NHP as manifested by
gadolinium uptake by the dura at the base of the temporal lobes. All animals
had measurable antibody in the CSF after invasion. These studies are the
first to demonstrate that experimental LNB in NHPs is a reliable model
faithful to the human disease, with spirochetal invasion of the subarachnoid
space. This also is the first report of CSF samples positive by culture in
experimental LNB. Inflammation in the CNS as manifested by CSF pleocytosis
and MRI findings was also correlated with the presence of spirochetal DNA
detected by PCR.

These data support the hypothesis that the pathogenesis of
LNB is associated with direct spirochetal invasion, and provide evidence
that CNS involvement is more common than heretofore thought.
Bb tunkeutuminen keskushermostoon on luultua yleisempää:

PMID: 7824109 [PubMed - indexed for MEDLINE]