Lähettäjä: Soijuv Lähetetty: 14.4.2006 9:32
Italialaiset tutkivat 1931 punkkia. N. 8 % oli Bb- , 4 % Ehrlichia-, 1,6 % rikettsia- ja babesiapositiivisia. Osa punkeista kantoi useita eri bakteereita.
http://www.ncbi.nlm.nih.gov/entrez/quer ... med_docsum
Vector Borne Zoonotic Dis. 2006 Spring;6(1):24-31.
A Study of the Presence of B. burgdorferi, Anaplasma (Previously Ehrlichia) phagocytophilum, Rickettsia, and Babesia in Ixodes ricinus Collected within the Territory of Belluno, Italy.
Piccolin G, Benedetti G, Doglioni C, Lorenzato C, Mancuso S, Papa N, Pitton L, Ramon MC, Zasio C, Bertiato G.
Observatory for the Study, Surveillance and Prevention of Tick-Borne Diseases, ULSS, Veneto, Belluno, Italy.
In the years 2000 and 2001, we sampled ticks in order to establish the distribution of Ixodes ricinus in the province of Belluno; 5987 tick samples from 244 sites throughout the province were gathered, by dragging for a 5-min period. In 40 sites, seasonal variations and cycle stages of the parasites were studied at monthly intervals from March to September. A polymerase chain reaction (PCR) technique was used to identify the tick-infected sites.
Of 1931 individual ticks, 8.23% were positive for Borrelia burgdorferi, 4.4% were positive for Ehrlichia, 1.6% were positive for Rickettsia, and 1.6% were positive for Babesia. The co-presence of Borrelia and Ehrlichia (1.2%) and Babesia (0.5%), Borrelia, Ehrlichia and Rickettsia (0.1%) was also found.
PMID: 16584324 [PubMed - in process]
PUNKEISSA USEITA ERI TAUDINAIHEUTTAJIA
Valvojat: Jatta1001, Borrelioosiyhdistys, Bb
PUNKEISSA USEITA ERI TAUDINAIHEUTTAJIA
Viimeksi muokannut Bb, Pe Maalis 06, 2009 23:49. Yhteensä muokattu 1 kertaa.
Lähettäjä: Soijuv Lähetetty: 14.4.2006 9:42
Saksalaiset keräsivät 2518 punkkia Bonnin puutarhoista ja puistoista. N. 18 % punkeista oli Bb-positiivisia; B.afzelii 39,5 %, B.garinii 27,9 %, Bbss 15,6 % ja B. valaisiana 8,6 %. Useita alalajeja löytyi 4,3 %:sta punkkeja, 4,3 %:a ei kyetty luokittelemaan.
Parasitol Res. 2005 Jan;95(1):5-12. Epub 2004 Nov 10.
Borrelia burgdorferi infection prevalences in questing Ixodes ricinus ticks
(Acari: Ixodidae) in urban and suburban Bonn, western Germany.
Maetzel D, Maier WA, Kampen H.
Institute for Medical Parasitology, University of Bonn, Sigmund Freud
Strasse 25, 53105 , Bonn, Germany.
From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus
ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket
dragging at 45 sites all over the city area of Bonn, western Germany, to be
checked for Borrelia burgdorferi infection. The collection sites included 20
private gardens, nine public recreational parks, the boundaries of 14
sylvatic suburban areas and two footpaths between suburban farmed fields.
Generally, numbers of specimens collected along sylvatic suburban areas and
at urban sites with dense tree populations were significantly higher than at
the other collection sites. Out of 1,394 specimens (865 nymphs, 241 females,
288 males) that were randomly chosen for Borrelia analysis by a simple PCR,
250 (17.9 %) were found to be infected with B. burgdorferi sensu lato. While
the infection prevalences varied significantly between females (26.6%),
males (12.5%) and nymphs (17.3%), there were no striking differences between
sylvatic and unwooded sites.
A total of 92.8% of the ticks Borrelia-positive
by the simple PCR were also positive in a diagnostic nested PCR. Using
genospecies-specific oligonucleotide probes, single Borrelia genospecies
infections (91.4%) could be assigned to B. afzelii (39.5%), B. garinii
(27.9%), B. burgdorferi sensu stricto (15.6%) and B. valaisiana (8.6%) by
DNA hybridization. Various combinations of double infections were observed
in 4.3% of the infected ticks. Another 4.3% of the Borrelia infections were
untypeable. The B. burgdorferi genospecies distribution in the city area was
shown to be variable from site to site and, even more, it was distinct from
rural collection sites near Bonn. This is ascribed to a different spectrum
of reservoir hosts.
Taking into account the infection prevalences of
host-seeking ticks in the forested surroundings of Bonn, our study
demonstrates that the risk of acquiring Lyme disease after a tick bite in
urban/suburban areas is comparably as high as in woodlands outside of the
city.
PMID: 15549390 [PubMed - indexed for MEDLINE]
Saksalaiset keräsivät 2518 punkkia Bonnin puutarhoista ja puistoista. N. 18 % punkeista oli Bb-positiivisia; B.afzelii 39,5 %, B.garinii 27,9 %, Bbss 15,6 % ja B. valaisiana 8,6 %. Useita alalajeja löytyi 4,3 %:sta punkkeja, 4,3 %:a ei kyetty luokittelemaan.
Parasitol Res. 2005 Jan;95(1):5-12. Epub 2004 Nov 10.
Borrelia burgdorferi infection prevalences in questing Ixodes ricinus ticks
(Acari: Ixodidae) in urban and suburban Bonn, western Germany.
Maetzel D, Maier WA, Kampen H.
Institute for Medical Parasitology, University of Bonn, Sigmund Freud
Strasse 25, 53105 , Bonn, Germany.
From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus
ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket
dragging at 45 sites all over the city area of Bonn, western Germany, to be
checked for Borrelia burgdorferi infection. The collection sites included 20
private gardens, nine public recreational parks, the boundaries of 14
sylvatic suburban areas and two footpaths between suburban farmed fields.
Generally, numbers of specimens collected along sylvatic suburban areas and
at urban sites with dense tree populations were significantly higher than at
the other collection sites. Out of 1,394 specimens (865 nymphs, 241 females,
288 males) that were randomly chosen for Borrelia analysis by a simple PCR,
250 (17.9 %) were found to be infected with B. burgdorferi sensu lato. While
the infection prevalences varied significantly between females (26.6%),
males (12.5%) and nymphs (17.3%), there were no striking differences between
sylvatic and unwooded sites.
A total of 92.8% of the ticks Borrelia-positive
by the simple PCR were also positive in a diagnostic nested PCR. Using
genospecies-specific oligonucleotide probes, single Borrelia genospecies
infections (91.4%) could be assigned to B. afzelii (39.5%), B. garinii
(27.9%), B. burgdorferi sensu stricto (15.6%) and B. valaisiana (8.6%) by
DNA hybridization. Various combinations of double infections were observed
in 4.3% of the infected ticks. Another 4.3% of the Borrelia infections were
untypeable. The B. burgdorferi genospecies distribution in the city area was
shown to be variable from site to site and, even more, it was distinct from
rural collection sites near Bonn. This is ascribed to a different spectrum
of reservoir hosts.
Taking into account the infection prevalences of
host-seeking ticks in the forested surroundings of Bonn, our study
demonstrates that the risk of acquiring Lyme disease after a tick bite in
urban/suburban areas is comparably as high as in woodlands outside of the
city.
PMID: 15549390 [PubMed - indexed for MEDLINE]