VIELÄ KYSTAMUODOSTA

[Bb]

Lähettäjä: jukka61 Lähetetty: 18.8.2004 7:54

Tässä on italialainen tutkimus "kystista":


APMIS. 2004 Jan;112(1):57-62.

*Induction of cystic forms by different stress conditions in Borrelia
burgdorferi.*

*Murgia R, Cinco M.*

Spirochete Laboratory, Dipartimento di Scienze Biomediche, Universita'
degli Studi di Trieste, Via Fleming 22, 34127 Trieste, Italy.

Cystic forms of Borrelia burgdorferi might represent a low metabolic
activity state or phase of B. burgdorferi cells that allows the
spirochete to survive in a hostile environment until conditions are
favourable to multiply again. In this study we evaluated the rate of
cyst formation induced by oxidative stress, pH variations, and heating,
reconversion of cysts to vegetative forms, and some aspects of their
metabolic activity. We observed cyst formation in the presence of
extreme pH values, and at high temperature, but the best production of
cystic forms was observed in the presence of H2O2. When transferred to
BSK II medium, the cystic forms reconverted to spirochetes in relation
to their age and type of induction treatment. Furthermore, we
demonstrated a low metabolic activity of cystic forms by measuring amino
acid incorporation. Overall, these data suggest that the phenomenon of
conversion to cysts by B. burgdorferi provides a limited survival
potential. This short-term survival, however, gives borreliae an
additional chance to overcome unfavourable environmental conditions.

PMID: 14961976 [PubMed - in process