Borrelioosia sairastavien ottamia mikroskooppikuvia borreliabakteerista.
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KUVIA BORRELIABAKTEERISTA
Valvojat: Jatta1001, Borrelioosiyhdistys, Bb
Punkinpuremasta saadun tartunnan jälkeen borreliabakteeri kulkeutuu ensin verenkiertoon ja "liftaa" sen jälkeen veren mukana eri puolille elimistöä. Siten se kulkeutuu mm sydämeen, niveliin ja aivoihin. Kanadalaistutkijat ovat nyt ensimmäistä kertaa nähneet 3D-kuvassa bakteerin etenemisen hiiren elimistössä. Tähän saakka tutkijoilla ei ole ollut selkeää kuvaa siitä miten bakteeri etenee elimistössä.
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Canadians first to illustrate Lyme disease bacterium
Updated Wed. Jul. 2 2008 4:27 PM ET
CTV.ca News Staff
Canadian researchers are the first in the world to use high-resolution, 3-D imaging to create dramatic new footage of the bacterium that causes Lyme disease as it moves through the bloodstream of a living host.
Microbiologists at the University of Calgary have been able to create a fluorescent strain of the bacterium Borrelia burgdorferi and observe its movement in mice.
B. burgdorferi is part of a group of bacteria known as pathogenic spirochetes, which cause a variety of bacterial diseases such as syphilis, leptospirosis and relapsing fever, as well as Lyme disease.
Until now, scientists have been unclear how these bacteria move from the blood to tissues in the body.
However, the ability to view the spirochete will allow scientists to better study and understand how these and other bacteria spread through the human body and lead to disease.
"When you get a tick bite, the spirochete gets into the blood and then hitchhikes through the body, gets out of the blood system and then gets into the heart, neurological tissue in the brain and in the joints," Dr. George Chaconas, one of the lead researchers, told CTV News. "So this is its way of getting transportation around the body." The image of the bacterium was published in the journal PLoS Pathogens.
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Abstract:
Real-Time High Resolution 3D Imaging of the Lyme Disease Spirochete Adhering to and Escaping from the Vasculature of a Living Host
Tara J. Moriarty, M. Ursula Norman, Pina Colarusso, Troy Bankhead, Paul Kubes, and George Chaconas
Pathogenic spirochetes are bacteria that cause a number of emerging and re-emerging diseases worldwide, including syphilis, leptospirosis, relapsing fever, and Lyme borreliosis. They navigate efficiently through dense extracellular matrix and cross the blood-brain barrier by unknown mechanisms. Due to their slender morphology, spirochetes are difficult to visualize by standard light microscopy, impeding studies of their behavior in situ. We engineered a fluorescent infectious strain of Borrelia burgdorferi, the Lyme disease pathogen, which expressed green fluorescent protein (GFP). Real-time 3D and 4D quantitative analysis of fluorescent spirochete dissemination from the microvasculature of living mice at high resolution revealed that dissemination was a multi-stage process that included transient tethering-type associations, short-term dragging interactions, and stationary adhesion. Stationary adhesions and extravasating spirochetes were most commonly observed at endothelial junctions, and translational motility of spirochetes appeared to play an integral role in transendothelial migration. To our knowledge, this is the first report of high resolution 3D and 4D visualization of dissemination of a bacterial pathogen in a living mammalian host, and provides the first direct insight into spirochete dissemination in vivo.
http://www.ctv.ca/servlet/ArticleNews/s ... hub=Health
Canadians first to illustrate Lyme disease bacterium
Updated Wed. Jul. 2 2008 4:27 PM ET
CTV.ca News Staff
Canadian researchers are the first in the world to use high-resolution, 3-D imaging to create dramatic new footage of the bacterium that causes Lyme disease as it moves through the bloodstream of a living host.
Microbiologists at the University of Calgary have been able to create a fluorescent strain of the bacterium Borrelia burgdorferi and observe its movement in mice.
B. burgdorferi is part of a group of bacteria known as pathogenic spirochetes, which cause a variety of bacterial diseases such as syphilis, leptospirosis and relapsing fever, as well as Lyme disease.
Until now, scientists have been unclear how these bacteria move from the blood to tissues in the body.
However, the ability to view the spirochete will allow scientists to better study and understand how these and other bacteria spread through the human body and lead to disease.
"When you get a tick bite, the spirochete gets into the blood and then hitchhikes through the body, gets out of the blood system and then gets into the heart, neurological tissue in the brain and in the joints," Dr. George Chaconas, one of the lead researchers, told CTV News. "So this is its way of getting transportation around the body." The image of the bacterium was published in the journal PLoS Pathogens.
-----------------------------------------------------------------------------
Abstract:
Real-Time High Resolution 3D Imaging of the Lyme Disease Spirochete Adhering to and Escaping from the Vasculature of a Living Host
Tara J. Moriarty, M. Ursula Norman, Pina Colarusso, Troy Bankhead, Paul Kubes, and George Chaconas
Pathogenic spirochetes are bacteria that cause a number of emerging and re-emerging diseases worldwide, including syphilis, leptospirosis, relapsing fever, and Lyme borreliosis. They navigate efficiently through dense extracellular matrix and cross the blood-brain barrier by unknown mechanisms. Due to their slender morphology, spirochetes are difficult to visualize by standard light microscopy, impeding studies of their behavior in situ. We engineered a fluorescent infectious strain of Borrelia burgdorferi, the Lyme disease pathogen, which expressed green fluorescent protein (GFP). Real-time 3D and 4D quantitative analysis of fluorescent spirochete dissemination from the microvasculature of living mice at high resolution revealed that dissemination was a multi-stage process that included transient tethering-type associations, short-term dragging interactions, and stationary adhesion. Stationary adhesions and extravasating spirochetes were most commonly observed at endothelial junctions, and translational motility of spirochetes appeared to play an integral role in transendothelial migration. To our knowledge, this is the first report of high resolution 3D and 4D visualization of dissemination of a bacterial pathogen in a living mammalian host, and provides the first direct insight into spirochete dissemination in vivo.